Table I. Proteins identified in the P2X7 receptor complex by MALDI-TOF MS and confirmed by western blotting.
| Protein name | Abbreviation | Molecular weight (kDa) | SwissProt/Trembl | Peptidesa |
|---|---|---|---|---|
| Extracellular | ||||
| laminin α3 chain | 187 | Q16787 | 7 (2, 3)b | |
| Transmembrane | ||||
| integrin β2 | 85 | P05107 | 9 | |
| protein tyrosine phosphatase β | RPTPβ | 222 | P23467 | 8 (2, 2)b |
| Intracellular | ||||
| β-actin | 42 | P02570 | 9 | |
| α-actinin 4 | 105 | O43707 | 6 | |
| heat shock cognate 71 kDa protein | Hsc71 | 71 | P11142 | 15 |
| heat shock 70 kDa protein 1 | Hsp70 | 70 | P08107 | 16 |
| heat shock protein HSP90-β | Hsp90 | 83 | P08238 | 9 |
| phosphatidylinositol 4-kinase 230 | PI4K | 231 | Q9UPG2 | 8 (3, 4)b |
| MAGUK P55 subfamily member 3 | MAGuK | 66 | Q13368 | 6 |
| supervillin | 201 | O60611 | 9 (3, 1)b |
aThe number of peptides matched following trypsin digestion.
bNumbers in parentheses indicate endo-Arg-C and endo-Asp-N digestions.