Fig. 5. Hsp90 binds to p53 that possesses wild-type conformation. Hsp90 (500 ng) or BSA (500 ng) was coated onto an ELISA plate as described in Materials and methods. The indicated amounts of wtp53, wtp53 pre-incubated for 90 min at 37°C or mutant p53R175H protein were then added in 50 µl of ELISA reaction buffer containing 1 mM ATP for 1 h at 25°C, followed by detection of p53 bound to Hsp90 as described in Materials and methods.