Fig. 4. AP-4 localization is affected by the expression of ARF1-GAP and GGA1 VHS-GAT. HeLa cells were transiently transfected with an ARF1-GAP expression plasmid (A–C) or a GGA1 YFP-VHS-GAT expression plasmid (D–F). After 24 h cells were fixed and stained using the anti-σ4 [Cy3 (A and D)] and anti-His₆ antibodies [Alexa 488 (B)]. Transfected cells are indicated by arrows. Bars, 5 µm.