Fig. 6. Interaction analyses of ε adaptin and µ4 adaptin with switch mutants of ARF1. (A and C) HF7C yeast strain was transfected with constructs expressing the indicated proteins and co-transformants were spotted on plates with (bottom) or without (top) histidine. Growth of colonies on the plate lacking histidine indicates an interaction of the proteins. (B) In vitro transcribed/translated ³⁵S-labeled ε and ARF-myc constructs were mixed, incubated, and ARF-myc was immunoprecipitated using an anti-myc antibody. Top, the co-precipitation of ε_1–727 was detected by autoradiography. Middle and bottom, labeled ε_1–727 and ARF1 constructs, respectively, which were used as input.