Fig. 5. Absence of Nog2p leads to an accumulation of the 27SB_S and 7S_S pre-rRNA. Total RNAs were extracted from the LMA159 strain after growth in YPGal or after shift to YPGlu for various times, as indicated. (A) Primer extensions were performed using primer CS10 (specific for the different 27S forms). The reaction products were resolved on a 5% acrylamide denaturing gel. (B) Northern blot analysis of 7S rRNA levels. The RNAs were separated on a 5% acrylamide denaturing gel, transferred to a nylon membrane and probed with oligonucleotide CS3, which hybridizes within the 7S region. (C) Quantification of the hybridization signals for the 5.8S_S and 5.8S_L mature rRNAs. The levels were normalized to the hybridization signals obtained with a U3 RNA-specific probe; 100% corresponds to the galactose-induced conditions.