Fig. 3.

Estradiol induces diurnal changes in GnRH neuron firing and pattern. (A and B) Representative examples of firing patterns are shown for GnRH neurons from OVX+E mice recorded in the a.m. (A) and in the p.m. (B). Data are plotted as mean firing rate at 1-min intervals. Vertical lines at the top of each graph illustrate the timing of the individual action currents detected. (C–F) Mean values for firing rate (C), median instantaneous frequency (D), maximum duration of quiescence (E), and percentage of time in quiescence (F). Open bars represent values from cells recorded in the a.m., and filled bars indicate values from cells recorded in the p.m. ***, P < 0.001.