Figure 5.
The function of the BREd is context-dependent. The core promoters indicated in A–E (wild type; wt) were tested in transcription assays alongside a derivative of each promoter that contained a mutated BREd sequence (df). The numbers below each panel are quantitation of the level of transcription relative to that observed at basal level for each wild-type promoter. Schematics of each promoter are shown at the right with the core promoter elements indicated; arrows depict the transcription start sites. The core promoters were compared in an in vitro transcription assay with HeLa nuclear extract in the absence or presence of the activator GAL4-AH (50 ng). Full details for each core promoter and specific mutations are shown in Supplementary Figure 2.