Table 1.
Summary of results from two separate experiments implanting CHO cells into SCID mice
| Implanted cells | Mean human IgG in sera (ng/ml) (no. of mice/group) | Terminal proteinuria (range for group) | Mean proteinuria (estimateda g/l) | Mean day of death |
| Experiment 1 | ||||
| CHO-B3 | <16 (3/5), 0 (2/5) | ++/+++ to +++ | 2.4 | 20 |
| CHO-B3(R27aS) | 260 (2/5), <16 (3/5) | + to ++ | 0.65 | 24 |
| Nontransfected CHO only | 0 | Trace to trace/+ | 0.2 | 34b,c |
| Pristane only | 0 | Trace/+ | 0.2 | 34b |
| Experiment 2 | ||||
| CHO-B3 | 105 (4/5), NR (1/5) | ++ to +++ | 2.4 | 26 |
| CHO-B3(R27aS) | 420 (5/5) | + to ++/+++ | 0.99 | 39 |
| CHO with empty vector | 0 | Trace to +/++ | 0.38 | 48 |
| Pristane only | 0 | Trace/+ | 0.2 | 56b |
aProteinuria was assessed using Albustix, which give a semiquantitative measure based on colour change. Based on values provided with the Albustix, in order to calculate the estimated proteinuria in g/l we assumed that trace = 0.1 g/l, trace/+ = 0.2 g/l, +/++ = 0.65 g/l, and ++/+++ = 2.0 g/l. The proteinuria data in both experiments were found to be normally distributed using the Kolmogorov–Smirnov test and compared using a one-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison test. In experiment 1, the ANOVA showed that there was a significant difference between the mean proteinuria values of all the groups (P < 0.0001). The Bonferroni post test indicated that there was a significant difference between mice implanted with CHO-B3 and CHO-B3(R27aS) (P < 0.001), nontransfected CHOdhfr- cells (P < 0.001), and pristane only (P < 0.001). In experiment 2, the ANOVA showed that there was a significant difference between the mean proteinuria values of all the groups (P < 0.0003). The Bonferroni post test indicated that there was a significant difference between mice implanted with CHO-B3 and CHO-B3(R27aS) (P < 0.05), CHO cells containing the empty vector (P < 0.001), and pristane only (P < 0.01). bMice still healthy at the end of the experiment. cThese cells did not survive/grow in the mice. CHO, Chinese hamster ovary; NR, not recorded; SCID, severe combined immunodeficiency.