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. 2005 Jun 22;7(5):R1014–R1022. doi: 10.1186/ar1771

Figure 2.

Figure 2

Western blot analysis of anti-TIMP-2 antibodies. Lysates of THP-1 (a human monocytic cell line) and H9 (a human T-cell lymphoma) were separated in 18% Tris-glycine gel, transferred into a polyvinylidene fluoride membrane, and blotted with immunoglobulin G (IgG) fractions from a patient with rheumatoid arthritis having high levels of anti-TIMP-2 antibodies detected by ELISA. The IgG fraction visualized a band of molecular weight 22 kDa, corresponding to TIMP-2. TIMP, tissue inhibitor of metalloproteinases.