Figure 2.

Five-week low dose ACTH_1–24 treatment produces a highly significant effect on bone formation by evaluation of calcein and xylenol orange labeling. The variability may reflect in part the ~10% variability in animal weights (see Materials and Methods), 4.2 ± 0.4 kg for each group.

A. Examples of calcein and xylenol orange labels at the center of femoral head in treated (top) and control (bottom) animals. To show the trabecular structure, right panels show the same fluorescent labels superimposed on phase images. Each image is 580 across x 750 microns high.

B. Comparison of labeled surfaces (double labels plus 1/2 of single labeled area)/ total area in control and treated has p = 0.0089. The results are in keeping with differences in the same animals by micro computed tomography (Fig 3, below).