Figure 5. PCR for osteoblast and osteoclast targets in bone marrow cells.

Cells were used to produce mRNA as described in methods. Significant increases in osteoblast products are seen (A-D) in keeping with effects of bone formation by labeling (Fig 2). In contrast, there are no significant differences in osteoclast products in keeping with lack of effects on bone degradation (E-H).

A. Alkaline Phosphatase expression (p = 0.033, n = 5).

B. Bone gla protein (osteocalcin) (p = 0.012, n = 5).

C. Type 1 collagen (p = 0.046, n = 5).

D. Bone morphogenetic protein 2 (p = 0.011, n = 5)

E. Tartrate-resistant acid phosphatase secreted by osteoclasts (not significant, n = 5).

F. Cathepsin K expressed highly in osteoclasts (not significant, n = 5).

G. ATPa3 subunit of the V-ATPase expressed in osteoclasts (not significant, n = 5).

H. ATPd2 subunit of the V-ATPase expressed in osteoclasts (not significant, n = 5).