Table 1.
Rabbit and Human PCR Primers
| Rabbit Primers | ||
| ALP - alkaline phosphatase | ||
| Forward | 5’-ACTGTGGACTACCTCTTG-3’ | |
| Reverse | 5’-GGTCAGTGATGTTGTTCC-3’ | Product size 76 bp |
| Col1A1 - collagen 1 alpha 1 subunit | ||
| Forward | 5’-CACATGCGTGCAGAACGGCG-3’ | |
| Reverse | 3’-CGCGTCTTCGGGGCAGACAG-5’ | Product size 184 bp |
| BMP2 bone morphogenetic protein 2 | ||
| Forward | 5’-GCTTCGACGTCACCCCTGCC-3’ | |
| Reverse | 3’-AGGGGGTGCCCCTTCCCATC-5’ | Product size 211 bp |
| TRAP (ACPA5) tartrate resistant acid phosphatase | ||
| Forward | 5’- GAAACCACGACCACATTGGC -3’ | |
| Reverse | 3’- GTCACCGTGTCCAGCATGTA -5’ | Product size 151 bp |
| BGLAP - bone gamma carboxyglutamate protein (osteocalcin) | ||
| Forward | 5’- GCTCAGCCTTCGTGTCCA -3’ | |
| Reverse | 3’- CCTGCCCGTCAATCAGTT -5’ | Product size 75 bp |
| ATPa3 (V-ATPase subunit a3) | ||
| Forward | 5’- CTGTTCGGCTACCTCGTGTT -3’ | |
| Reverse | 3’- TAGGACGGACTGGACCACTT -5’ | Product size 171 bp |
| ATPd2 (V-ATPase subunit d2) | ||
| Forward | 5’- CGCTCAGCACCTTTTTCACC -3’ | |
| Reverse | 3’- CTAGCGGTGTTTCCACCAGT -5’ | Product size 207 bp |
| CTSK (cathepsin K) | ||
| Forward | 5’- TCAAAGTACCCCCGTCTCGT -3’ | |
| Reverse | 3’- TGAAAGCCCAACAGGAACCA -5’ | Product size 150 bp |
| Human Primer | ||
| Col1A1 - collagen type I alpha 1 chain | ||
| Forward | 5’-AGGGACACAGAGGTTTCAGTGGTT-3’ | |
| Reverse | 5’- GCAGCACCAGTAGCACCATCATTT-3’ | Product size 198 bp |
| RunX2 – RUNX family transcription factor 2 | ||
| Forward | 5’-TGGTTACTGTCATGGCGGGTA-3’ | |
| Reverse | 5’-TCTCAGATCGTTGAACCTTGCTA-3’ | Product size 101 bp |
| ALPL – alkaline phosphatase, biomineralization associated | ||
| Forward | 5’-ACCACCACGAGAGTGAACCA -3’ | |
| Reverse | 5’-CGTTGTCTGAGTACCAGTCCC -3’ | Product size 79 bp |
| BGLAP - bone gamma-carboxyglutamate protein (Osteocalcin) | ||
| Forward | 5’- CACTCCTCGCCCTATTGGC -3’ | |
| Reverse | 5’- CCCTCCTGCTTGGACACAAAG -3’ | Product size 112 bp |
| VEGF - vascular endothelial growth factor A | ||
| Forward | 5’- AAGGAGGAGGGCAGAATCAT -3’ | |
| Reverse | 5’- ATCTGCATGGTGATGTTGGA -3’ | Product size 225 bp |
| FLT1 - fms related receptor tyrosine kinase 1 (VEGFR1) | ||
| Forward | 5’- TTTGCCTGAAATGGTGAGTAAGG -3’ | |
| Reverse | 5’- TGGTTTGCTTGAGCTGTGTTC -3’ | Product size 117 bp |
| FLK1/KDR - kinase insert domain receptor (VEGFR2) | ||
| Forward | 5’- GGCCCAATAATCAGAGTGGCA -3’ | |
| Reverse | 5’- CCAGTGTCATTTCCGATCACTTT -3’ | Product size 109 bp |
| MC2R – melanocortin 2 (adrenocorticotropic hormone) receptor | ||
| Forward | 5’- GACTGTCCTCGTGTGGTTTTG -3’ | |
| Reverse | 5’- GGCTGCCCAGCATATCAGAT -3’ | Product size 166 bp |
| NDUFA5 – NADH: ubiquinone oxidoreductase subunit A5 | ||
| Forward | 5’- GAGAAGCTGGCTATGGTTAAAGCG -3’ | |
| Reverse | 5’- CCACTAATGGCTCCCATAGTTTCC -3’ | Product size 154 bp |
| NDUFB1 – NADH: ubiquinone oxidoreductase subunit B1 | ||
| Forward | 5’- GTCCCTATGGGATTTGTCATTGG -3’ | |
| Reverse | 5’- CAGTTAGCCGTTCATCACTCTT -3’ | Product size 60 bp |
| NDUFB6 – NADH: ubiquinone oxidoreductase subunit B6 | ||
| Forward | 5’- CCACAGAAGATGGGGCCTATG -3’ | |
| Reverse | 5’- TCCAGACAGGTACAAGTACATGA -3’ | Product size 133 bp |
| NDUFS2 – NADH: ubiquinone oxidoreductase subunit S2 | ||
| Forward | 5’- ACCCAAGCAAAGAAACAGCC -3’ | |
| Reverse | 5’- AATGAGCTTCTCAGTGCCTC -3’ | Product size 214 bp |
| BMP2 – bone morphogenetic protein 2 | ||
| Forward | 5’- ACTACCAGAAACGAGTGGGAA -3’ | |
| Reverse | 5’- GCATCTGTTCTCGGAAAACCT -3’ | Product size 113 bp |
| TGFβ – transforming growth factor, beta 1 | ||
| Forward | 5’- CTAATGGTGGAAACCCACAACG -3’ | |
| Reverse | 5’- TATCGCCAGGAATTGTTGCTG -3’ | Product size 209 bp |
| SMAD1 - SMAD family member 1 | ||
| Forward | 5’- TTGGCACAGTCTGTGAACCATGG -3’ | |
| Reverse | 5’- GTAACATCCTGGCGGTGGTATTC -3’ | Product size 116 bp |
| SMAD2 - SMAD family member 2 | ||
| Forward | 5’- GGGTTTTGAAGCCGTCTATCAGC -3’ | |
| Reverse | 5’- CCAACCACTGTAGAGGTCCATTC -3’ | Product size 149 bp |
| SMAD3 - SMAD family member 3 | ||
| Forward | 5’- TGAGGCTGTCTACCAGTTGACC -3’ | |
| Reverse | 5’- GTGAGGACCTTGTCAAGCCACT -3’ | Product size 156 bp |
| WNT1 – wingless-type MMTV integration site family, member 1 | ||
| Forward | 5’- CTCTTCGGCAAGATCGTCAACC -3’ | |
| Reverse | 5’- CGATGGAACCTTCTGAGCAGGA -3’ | Product size 112 bp |
| β-Catenin – catenin beta 1 (CTNNB1) | ||
| Forward | 5’- CGTGGACAATGGCTACTCAAGC -3’ | |
| Reverse | 5’- TCTGAGCTCGAGTCATTGCATAC -3’ | Product size 285 bp |
| BGN- Biglycan | ||
| Forward | 5’- GAGACCCTGAATGAACTCCACC -3’ | |
| Reverse | 5’- CTCCCGTTCTCGATCATCCTG -3’ | Product size 131 bp |
| ITGA5 - Integrin α5 | ||
| Forward | 5’- GCCGATTCACATCGCTCTCAAC -3’ | |
| Reverse | 5’- GTCTTCTCCACAGTCCAGCAAG -3’ | Product size 139 bp |
| ITGB1 - Integrin β1 | ||
| Forward | 5’- GGATTCTCCAGAAGGTGGTTTCG -3’ | |
| Reverse | 5’- TGCCACCAAGTTTCCCATCTCC -3’ | Product size 143 bp |