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Fig. 6. Western blot analysis after SDS–PAGE separation of mitochondrial membranes (20 µg) from yeasts expressing wild-type or double and triple mutants of CYT1. Residues ligating the L5 (CL) phosphodiester group A have been mutated: (K288L/K289L, K288L/K296L, K289L/K296L and K288L/K289L/K296L). RIP1, COB and CYT1 were detected using specific antibodies.
