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. 2005 Oct 20;115(11):3057–3071. doi: 10.1172/JCI24792

Figure 7.

Figure 7

Prevention and treatment of oxazolone-colitis with NF-κB decoy ODNs — effect on cytokine production and intracellular proteins. (A) Cytokine production in cultures of colonic LPMCs isolated on day 3 after oxazolone administration. Cytokine concentrations were determined in culture supernatant by ELISA. Data shown are mean values ± SD and are representative of 2 independent experiments. (B) Monocyte-derived chemokine/CCL22 (MDC/CCL22) production in ex vivo colon cultures isolated on day 3 after oxazolone administration; chemokine concentrations in the culture supernatant were determined by ELISA. Data shown are mean values ± SD and are representative of 2 independent experiments. (C) Cytokine production in cultures of colonic LPMCs on day 7 after oxazolone administration and day 3 after oligonucleotide administration; each culture contained cells pooled from at least 3 mice; cytokine concentrations were determined in culture supernatant by ELISA. (D) DNA-binding activity of p65 in nuclear extracts of cells derived from lamina propria on day 3 after oxazolone administration measured in nuclear extracts from colonic lamina propria cells by TransFactor assay. (E) IRF4 protein expression is reduced in colonic LPMCs. Total colonic LPMC lysates were analyzed by Western blotting on day 3 after oxazolone administration. *P < 0.01. n.d., not detectable.