Table I. Growth of WAM121 containing plasmid-borne ffh alleles.

Allele	Vector
	pHDB6	pHDB1
Wild type	+	+
G110S	–	+
P142L	–	+
A192D	–	+
L195P	–	+
D253N	+	n.d.
A254L	+	n.d.
R255N	+	n.d.
G256A	+	n.d.
G257A	–	–

Cells transformed with pHDB6 and pHDB1 derivatives were streaked on LB agar containing 200 µM IPTG and plain LB agar, respectively. +, wild-type colony size; –, little or no growth; n.d., not done.