Fig. 7. Phagocytosis of yeast particles in wild-type and CRT/CNX double-null mutants. (A) Internalization of particles over time in two experiments (open and closed symbols) by wild-type AX2 cells (diamonds) and cells of two independent double mutants HG1772 (squares) and HG1773 (triangles). Particle uptake is plotted in arbitrary fluorescence units reflecting the number of internalized yeast particles. (B–E) Internalized particles distinguished by red fluorescence from adherent particles whose fluorescence is quenched. Phase-contrast images are colored in blue. Wild-type cells (B and C) show numerous ingested particles. In double-null cells (D and E), only few internalized particles are found. Arrowheads in (E) point to adherent particles not enveloped by a phagocytic cup. Bars for the upper and lower panels, 10 µm.