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Fig. 6. N-ethylmaleimide-treated MuB and ATPase-deficient MuB can stimulate transpososome assembly. (A) The time course of the assembly reaction at 5 nM Mu end in the presence or absence of 280 nM untreated or NEM-treated MuB is shown. (B) Mutant MuB defective in the ATPase activity (K106A and E174Q) can stimulate transpososome assembly.
