Fig. 8. Fusion of the Sys1p tail to the ER-resident protein Wbp1p leads to redistribution of the fusion protein in a (DXE) motif-dependent fashion. Cleared lysates of the protease-deficient strain cl3-ABYS-86, untransformed (A) or transformed with a recombinant plasmid expressing the indicated Wbp1-Sys1p tail fusion proteins (B and C), were subjected to sucrose gradient centrifugation. Proteins of different fractions were probed with antibodies specific for the ER marker Kar2p, the Golgi protein Emp47p and Wbp1p. Note the shift of the Wbp1p–Sys1 fusion protein with regard to its (DXE)-mutated version and to Wbp1 wild-type protein.