Fig. 1.

Snx2 mRNA is more abundant than Snx1 mRNA in extraembryonic yolk sacs at midgestation. (A) RT-PCR. Snx1, Snx2, and Hβ58 were amplified by RT-PCR from a litter of E8.5 wild-type embryos or their yolk sacs. (-RT) indicates mock RT reactions in which no reverse transcriptase was used. Equal amounts of +/-RT templates were used in each PCR, and identical amplification conditions were used for all samples. This experiment was repeated three times, and the quantitative averages of the data reveal that Snx2 mRNA is expressed at ≈72% of Snx1 mRNA levels in the embryo, but Snx2 mRNA levels are ≈225% of Snx1 mRNA levels in the yolk sac. (B) PCR amplification of Snx1 and Snx2 cDNA for assessing primer pair amplification ability. Snx1 or Snx2 cDNAs (0.15 ng) were PCR amplified with their gene-specific primers for varying numbers of cycles to demonstrate the comparable ability of the primers to amplify equal amounts of template.