Fig. 3.

Ci-CesA is the gene responsible for the sj mutation. (A) Expression of Ci-CesA was not detected in a sj mutant (sj), whereas the expression is evident at the trunk of a normal larva at the same age (normal). (Bar, 100 μm.) (B) Microinjection of MO specific to Ci-CesA caused the same phenotype as the sj mutant. A larva (Left) that developed from an egg into which Ci-CesA MO was injected showed the abnormal tunic, retraction of papillae, rotation of body axis, and stalk formation. A larva injected with control MO did not show such phenotypes (Right). (C) GFP expression from pCesA(-2080)-GFP and pCesA(-327)-GFP. (D) The reduction in cellulose in the sj mutant larva (sj), compared with the presence of cellulose in a normal larva at the same age (normal), as shown by dark blue signals.