Fig. 1. Toeprint analysis of 48S complexes assembled on wt GDVII RNA in the presence of increasing amounts of PTB and nPTB. Reactions were assembled with translation components as indicated in the table above the gel; molar ratios of PTB- and nPTB-dimers to mRNA are listed. Reference lanes T, A, C and G depict the negative-strand sequence and the position of the initiator AUG is indicated to the left. Positions of cDNA products terminated due to 48S complex formation are labeled as 48S to the right. Other RT stops that changed in intensity in the presence of PTB and nPTB are labeled by an asterisk on the right.