Fig. 1.

siRNA screen for genes synthetically lethal with MYC activation in the presence of suboptimal doses of DR5 agonists. (a) Screen schematic. Arrayed siRNAs were reverse-transfected into HA1E or HA1E-MYC cells and assayed for sensitivity to DR5-A (see Methods). (b) Genotype-dependent effects of siRNAs on cell viability. siRNAs were ranked in increasing order on the x axis by their ability to sensitize HA1E-MYC cells to DR5-A-induced apoptosis relative to the sensitization of HA1E cells (see Supporting Methods). The siRNA for GSK3β (siGSK3β) is indicated.