Fig. 2.

a HRMECs were treated with L-lactate (20 mM). The levels of classic angiogenic factors in HRMECs were measured via western blotting (WB) in the control and L-lactate groups (n = 3 per group). N, no significance; **p < 0.01. b Lactylation levels in HRMECs in the control and L-lactate (20 mM) groups were measured via WB. c HRMECs were treated with AZD3965 (8 nM) under hypoxic conditions. The levels of classic angiogenic factors in HRMECS were measured via WB in the control and AZD3965 groups (n = 3 per group). N, no significance; **p < 0.01. d Lactylation levels in HRMECs in the control and AZD3965 (8 nM) groups were measured via WB. e The angiogenic capacity of HRMECs was evaluated via a sprouting assay. Representative images of sprouting in the control and L-lactate (20 mM) groups (n = 3 independent experiments, 3 images for each group); Scale bar: 50 µm. **p < 0.01. f Representative images of spheroid sprouting in the control and AZD3965 (8 nM) groups (n = 3 independent experiments, 3 images for each group); Scale bar: 50 µm. **p < 0.01. g Migration was evaluated using a Transwell assay. Representative images in the control and L-lactate (20 mM) groups (n = 3 independent experiments, 3 images for each group); Scale bar: 50 µm. **p < 0.01. h Representative images of cell migration in the control and AZD3965 (8 nM) groups (n = 3 independent experiments, 3 images for each group); Scale bar: 50 µm. *p < 0.05. i Proliferation was evaluated via EdU staining. Representative images of proliferation in the control and L-lactate (20 mM) groups (n = 3 independent experiments, 3 images for each group); Scale bar: 100 µm. *p < 0.05. j Representative images of proliferation in the control and AZD3965 (8 nM) groups (n = 3 independent experiments, 3 images for each group); Scale bar: 100 µm. ***p < 0.001