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RRM2 is required for SF2/ASF to stimulate mRNA translation in vivo. (A) Schematic of the conserved heptapeptide present in RRM2 of SF2/ASF and point mutations analyzed. (B) In vivo translation assay comparing the activity of ΔRS and ΔRS (WQD-AAA) proteins. Western blot analysis of the transiently transfected constructs with anti-T7 monoclonal antibody (Lower). (C) In situ UV crosslinking mRNP-capture assay comparing cytoplasmic mRNA binding of SF2/ASF ΔRS protein with the RRM2 variant.
