Fig. 3. The glutamate auxotrophy of an rtg2Δ mutation is rescued by the lst8-5 but not the lst8-1 mutation. Wild-type PSY142 cells, and rtg2Δ, lst8-5, rtg2Δ lst8-5, rtg2Δ rtb2-5 rtg3Δ, lst8-1 and rtg2Δ lst8-1 derivatives were streaked on YNBD medium supplemented or not with 0.02% glutamate as indicated, and with uracil, leucine and lysine, and incubated at 30°C for 2–3 days.