Fig. 3. Methanandamide blocks native T and HVA currents of NG108-15 cells while WIN only affects HVA currents. (A) Effects on T-currents of 1 µM methanandamide applied first (2) followed by 1 µM WIN (3). (B) Effects on T currents of 1 µM WIN applied first (2) and 1 µM methanandamide applied after (3). (C and D) Corresponding current–voltage relationships (I–V curves) of the calcium currents presented in (A) and (B), respectively. Drugs were applied at HP –80 mV while I–V curves were performed just after at HP –110 mV in order to record maximal T-currents. (E and F) Percentage block of T- and HVA currents by 1 µM methanandamide (E) and by 1 µM WIN (F). To avoid contamination of T currents by HVA currents (and reciprocally), the peak of T-currents was measured at –30 mV while HVA currents were measured 100 ms after the beginning of the test pulse at +10 mV. In all experiments, HP was –80 mV and 10 mM Ba²⁺ was used as charge carrier.