Table 1.
The characteristics of approved studies for analysis.
| References | Melatonin dosage and supplementation stage | Groups and sample size | Included outcomes | Excluded outcomes |
|---|---|---|---|---|
| Takada et al, 2010 [40] | 10−9 M IVM |
ON1 = 209 ON2 = 171 One group |
1. Nuclear maturation 2. Cleavage rate 3. Blastocyst rate 4. DNA damage in blastocysts |
1. Data of no gonadotropin supplementation of media |
|
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| Farahavar et al, 2010 [41] | 10−4–10−8 M IVM |
ON1 = 370 ON2 = 135 Three groups |
1. Maturation rate 2. Cumulus expansion |
|
|
| ||||
| El-Raey et al, 2011 [7] | 4 × 10−7–4 × 10−8 M IVM |
ON1 = 360 ON2 = 114 Three groups |
1. Maturation rate 2. Cumulus expansion 3. Mitochondrial activity and distribution pattern 4. Steroidogenesis of COCs 5. ROS levels in oocytes |
1. Relative transcription of some genes not pertinent to this study 2-Data related to hydrogen peroxide |
|
| ||||
| Sampaio et al, 2012 [42] | 10−6–10−13 M IVM |
ZN1 = 415 ZN2 = 75 Six groups |
1. Cleavage rate 2. Blastocyst rate |
1. Autoradiographic binding assay of the melatonin receptors and NQO2 enzyme |
|
| ||||
| Takada et al, 2012 [43] | 10−9 M IVM |
ZN1 = 208 ZN2 = 209 One group |
1. Cleavage rate 2. Blastocyst rate 3. Hatching/hatched blastocyst 4. DNA damage in cumulus cells |
1. Data of media supplementation with melatonin without gonadotrophins |
|
| ||||
| Cebrian-Serrano et al, 2013 [44] | 10−3–10−12 M IVM | ZN1 = 1104 ZN2 = 602 Four groups |
1. Cleavage rate 2. Blastocyst rate 3. Total cell number of blastocysts |
1. Data of heat-stressed groups |
|
| ||||
| Tian, 2014 [34] | 10−3–10−11 M IVM |
ZN1 = 1581 ZN2 = 461 Six groups |
1. Oocyte maturation 2. Embryo development 3. Total cell number of blastocysts 4. Relative transcription in oocytes |
1. Data of melatonin receptor detection in COCs by immunofluorescence and Western blotting 2. Melatonin in bovine follicular fluid 3. Data of luzindole treatment |
|
| ||||
| Ahmed et al, 2016 [45] | 10−9 M IVM |
ON1 = 80 ON2 = 80 One group |
1. Maturation rate 2. Cumulus expansion 3. COC diameter |
1. Data of other supplements and heat-shock groups |
|
| ||||
| Tsantarliotou et al, 2007 [46] | 10−3–10−5 M IVM | ZN1 = 439 ZN2 = 155 Three groups |
1. Cleavage rate 2. Blastocyst rate |
|
|
| ||||
| Rodrigues-Cunha et al, 2016 [47] | 10−6, 10−9 M IVM |
ON1 = 136 ON1 = 68 Two groups |
1. Maturation rate 2. Relative transcription in oocytes and cumulus cells 3. Nuclear fragmentation in cumulus cells 4. ROS in oocytes 5. Cleavage rate 6. Blastocyst rate 7. Hatching rate |
1. Data of maturation at 6, 12, and 18 h and negative control of no gonadotropin supplementation of media |
|
| ||||
| Remiao et al, 2016 [48] | 10−6–10−12 M, IVM |
ON1 = 153 ON2 = 51 Three groups |
1. Viability 2. Maturation rate 3. Cleavage rate 4. Blastocyst rate 5. ROS and GSH in oocytes 6. Total cell number and apoptosis in blastocysts 7. Relative transcript in blastocysts |
1. The data of melatonin-loaded lipid-core nanocapsules |
|
| ||||
| Marques et al, 2018 [19] | 10−7–10−11 M, IVM and IVC |
ZN1 = 975 ZN2 = 470 Four groups (IVM) ZN1 = 895 ZN2 = 437 Four groups (IVC) |
1. GSH, ROS, and MMP in oocytes 2. Cleavage rate 3. Blastocyst rate and quality 4. Total cell number and apoptotic cells of blastocysts 5. GSH and ROS in blastocysts 6. Relative transcription in blastocysts |
1. Data on supplementation throughout the IVP process |
|
| ||||
| Wang et al, 2017 [49] | 10−11–3 × 10−11 M IVM |
ON1 = 798 ON2 = 251 Three groups |
1. Maturation rate of oocytes 2. Steroid secretion of COCs in spent medium 3. Relative transcription in COCs 4. ROS level in embryos 5. Development of androgenetic embryos (cleavage, morula, and blastocyst rates) |
1. Development of bovine parthenogenetic embryos (cleavage, morula, and blastocyst rates) 2. Melatonin receptors in embryos |
|
| ||||
| Zhao et al, 2018 [32] | 10−7–10−11 M IVM |
ON1 = 10,069 ON2 = 3852 Three groups |
1. Cytoplasmic maturation of MII oocytes (redistribution of mitochondria, cortical granules, and ER) 2. Intracellular glutathione (GSH) and ATP levels 3. Relative transcription in oocytes 4. Development results |
1. Western blot data 2. Receptors 3. Luzindole group 4. IP3R1 distribution and expression of CD9 and Juno |
|
| ||||
| Pang et al, 2018 [20] | 10−9 M IVM |
ON1 = 513 ON2 = 178 Three groups |
1. Nuclear and cytoplasmic maturation 2. Blastocyst rate and apoptosis in blastocysts 3. Relative transcription in embryos |
|
|
| ||||
| An et al, 2019 [50] | 10−5–10−11 M IVM |
ON1 = 965 ON2 = 276 Four groups |
1. Nuclear maturation of oocytes 2. Oxidative stress and cytoplasmic events 3. Development of embryos |
1. Epigenetic modifications in embryos 2. RNA sequencing data 3. Data of SCNT |
|
| ||||
| Cavallari et al, 2019 [51] | 10−6 M IVM |
ZN1 = 415 ZN2 = 415 One group |
1. Cleavage rate 2. Blastocyst rate 3. ROS level in oocytes |
1. Data of heat-shock and pro-oxidant treatment groups |
|
| ||||
| El-Sheikh et al, 2019 [52] | 10−7–10−9 M IVM |
ZN1 = 600 ZN2 = 200 Three groups |
1. Cleavage rate 2. Blastocyst rate |
1. Data of SH6-treated oocytes |
|
| ||||
| Lira et al, 2020 [53] | 10−7–10−11 M IVM |
ON1 = 340 ON2 = 116 Three groups |
1. Maturation rate 2. Cleavage rate 3. Morula rate 4. Blastocyst rate |
|
|
| ||||
| Gutierres-Anez et al, 2021 [54] | 10−11 M IVM |
ZN1 = 209 ZN2 = 201 One group |
1. Cleavage rate 2. Blastocyst rate 3. Total embryonic cells (ICM and TE) |
1. The data of season, donor category, and the interaction of donor category by season effect on the cumulus–oocyte complexes yield in adult cows and prepubertal dairy cattle |
|
| ||||
| Yaacobi-Artzi et al, 2020 [55] | 10−7 M IVM |
ON1 = 313 ON2 = 398 Two groups |
1. Cumulus expansion 2. Maturation rate 3. Cleavage rate 4. Blastocyst rate |
1. Data of heat-shocked groups |
|
| ||||
| Su et al, 2021 [56] | 10−7 -10−10 M IVM |
ON1 = 1552 ON2 = 443 Five groups |
1. Maturation rate 2. ROS levels in oocytes |
1. Data of sperm 2. Data after fertilization |
|
| ||||
| Cordova et al, 2022 [57] | 2 × 10−7M IVM |
NA | 1. Relative transcription in cumulus cells and blastocysts | 1. The development outcomes and quality of parthenogenetic blastocysts 2. Data of EGF supplement to medium 3. Immunofluorescence analysis |
|
| ||||
| Tutt et al, 2023 [58] | 10−7 M IVM |
ZN1 = 105 ZN2 = 105 One group |
1. The outcomes of embryo development | 1. The results of media without CP 2. RNA-Seq analyses 3. Spent media analyses 4. DNA methylation, chromosomal errors, and aneuploidy in Day 8 blastocysts |
|
| ||||
| Cheuqueman et al, 2015 [59] | 10−6–10−11 M IVF |
NA | 1. Cleavage rate 2. Blastocyst rate 3. Total cell count |
1. Data of sperm analysis |
|
| ||||
| Papis, 2007 [60] | 10−4 M IVC (2 days) |
ZN1 = 100 ZN2 = 101 One group |
1. Blastocyst rate of zygotes 2. Blastocyst rate of four-cell embryos |
1. Data of culture at atmospheric oxygen |
|
| ||||
| Wang et al, 2014, a [61] | 10−3–10−11 M IVC |
ZN1 = 1481 ZN2 = 681 Seven groups BN1 = 315 BN2 = 63 Five groups |
1. Cleavage rate 2. Blastocyst rate 3. Hatched blastocysts 4. Total cell number of blastocysts 5. Relative transcription in blastocysts |
1. The data of melatonin receptors 2. The data of luzindole-treated groups |
|
| ||||
| Wang et al, 2014, b [33] | 10−7 M IVC |
ZN1 = 118 ZN2 = 117 One group |
1. Cleavage rate 2. 8-cell stage rate 3. Blastocyst rate |
1. Hatched-blastocyst rate of vitrified embryos 2. Relative transcription in vitrified blastocysts |
|
| ||||
| Komninou, 2016 [62] | 10−6–10−12 M IVC |
ZN1 = 453 ZN2 = 165 Three groups |
1. Cleavage rate 2. Blastocyst rate 3. Hatched blastocysts 4. Total cell number of blastocysts 5. ROS level and apoptosis in blastocysts 6. Relative transcription in blastocysts |
1. Data of nanocapsule-coated groups |
|
| ||||
| Hitit et al, 2023 [63] | 10−3–10−9 M IVC |
BN1 = 1846 BN2 = 709 Five groups |
1. Blastocyst rat at D8 and D9 2. Hatched blastocyst at D9 3. Blastocyst cell number 4. Caspase-3/7 activity 5. Fragmentation of blastomeres |
1. Data of heat-stressed embryos and groups of atmospheric oxygen tension |
Note: ON1, number of oocytes in treatment; ON2, number of oocytes in control; ZN1, number of presumptive zygotes in treatment; ZN2, number of presumptive zygotes in control; BN1, number of blastocysts in treatment; BN2, number of blastocysts in control; GSH, glutathione; TE, trophectoderm.
Abbreviations: CPs, complex proteins; EGF, epidermal growth factor; ER, endoplasmic reticulum; ICM, inner cell mass; IVP, in vitro production; MMP, mitochondrial membrane potential; NA, not applicable; ROS, reactive oxygen species; SCNT, somatic cell nuclear transfer.