Fig. 5. The effect of non-lamellar lipids (DOPE) on the osmotic activation of OpuA. The proteoliposomes were composed of 38 mol% DOPG and 6 (circles), 13 (squares), 25 (upright triangles), 38 (inverted triangles) or 50 mol% DOPE (diamonds) plus 56, 49, 37, 24 or 12 mol% DOPC, respectively, and were obtained via fusion of proteoliposomes (DOPC/DOPE/DOPG, 2:1:1 mole ratio) with liposomes of the appropriate composition in a 1:3 mole ratio. Experimental details are as described in the legend to Figure 4.