Fig. 2. Translesion synthesis by in vitro synthesized and purified hPolη on templates containing site-specific lesions in the same sequence context. Bypass across six different lesions within the same template sequence was measured in primer extension assays containing hPolη synthesized in vitro (A) or hPolη polymerase domain purified from E.coli (B). The template contained either no lesion, 8-oxo-G, O⁴-MeT, O⁶-MeG, an abasic site or ethenoA. The sequence of the single-stranded template is indicated with an asterisk marking the position of the lesion. Controls, both without expression vector, were reticulocyte lysate in (A) and purified cell extract in (B).