Fig. 8. Translesion synthesis by purified wild-type and S62G hPolη proteins on an unmodified template and a template containing 8-oxo-G. Primer extension and bypass of a site-specific 8-oxo-G lesion were analyzed in assays containing 0.1 pg/µl (2 nM) of purified wild-type or S62G polymerase domain. The time course of incorporation over 30 min is shown on (A) unmodified and (B) 8-oxo-G-containing template primers. The template lesion (∗) was three nucleotides downstream of the 3′ primer terminus (arrow). Initial rates of incorporation in assays containing a 2-fold higher concentration of undamaged template primer (C) were quantitated by phosphoimage analysis (D).