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. 2005 Oct 19;33(18):5887–5895. doi: 10.1093/nar/gki889

Figure 1.

Figure 1

(A) UV-melting curves for the pyrene-modified RNA oligonucleotide duplex, I/VIII (circles) and unmodified RNA duplex, VI/VIII (triangles). (B) UV-melting curves for the pyrene-modified DNA oligonucleotide duplex, II/IX (circles) and unmodified DNA duplex, VII/IX (triangles). Measurements were carried out at 260 nm for a 1:1 mixture of oligonucleotides (33 µM) in a pH 7 buffer containing 10 mM sodium phosphate and 100 mM NaCl.