Figure 3.

(A) Steady-state fluorescence spectra for pyrene-modified RNA oligonucleotide, I (thin line) and its duplex with RNA, I/VIII (bold line). (B) Steady-state fluorescence spectra for pyrene-modified DNA oligonucleotide, II (thin line) and its duplex with RNA, II/IX (bold line). The measurements were carried out at room temperature for a 1:1 mixture of oligonucleotides (6 µM) in the same buffer solutions used in Figure 2. The excitation wavelength was 350 nm.