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. 2002 Feb 1;21(3):294–302. doi: 10.1093/emboj/21.3.294

graphic file with name cdf042f2.jpg

Fig. 2. N2 molecule traced after sD1–Fc and fD1 stimulations. BaF3 was stimulated for 1.5 h under the conditions indicated in the figure and then separated into membrane/cytosol-rich and nucleus-rich fractions. In each fraction, N2 fragments containing an ICD were analyzed by western blot analysis using the bhN6 antibody after immunoprecipitation with an anti-N2 polyclonal antibody.