. 2025 Sep 29;13(11):e00018-25. doi: 10.1128/spectrum.00018-25

TABLE 1.

Binding kinetics of monoclonal antibodies against target antigen, recombinant leptospiral virulence-modifying protein, LA0591^a^,^b^,^c^,^d^,^e

Clones	Ab loading response in nm shift	Con. (nM)	Ag binding response in nm shift	KD (M)	k_on (1 /Ms)	k_dis (1 /s)	Full X²	Full R²
6A5	0.2086	500	0.1109	<1.0E-12	2.05E+04	<1.0E-07	0.0085	0.9742
5G10	1.1788	500	0.2255	<1.0E-12	7.54E+03	<1.0E-07	0.0324	0.9884
5F8	1.2214	500	0.3633	1.41E-09	9.84E+03	1.38E-05	0.0059	0.999
5E10	0.891	500	0.3176	4.19E-09	1.26E+04	5.27E-05	0.0076	0.998
5A7	1.1815	500	0.1297	5.92E-08	5.17E+03	3.06E-04	0.0036	0.995

^{^a}

KD (M): Equilibrium dissociation constant.

^{^b}

k_on, on-rate constant, and k_dis, dissociation-rate constant, which have units of M⁻¹ s⁻¹ and s⁻¹, respectively.

^{^c}

This statistic quantifies the overall difference between a model's predictions and the observed experimental data.

^{^d}

A smaller "Full X²" value indicates a closer alignment of the model with the data.

^{^e}

A higher "Full R²" value signifies that the model explains a greater proportion of the observed variability.