TABLE 2.
Biolayer interferometry (Octet) analysis of monoclonal antibody pairing using as binding antigen recombinant leptospiral virulence-modifying protein, LA0591a,b,c,d
| Capture mAb in pair | Detection mAb in pair | ||||
|---|---|---|---|---|---|
| 6A5 | 5E10 | 5G10 | 5F8 | 5A7 | |
| 6A5 | 0.04 | 0.3246 | 0.2652 | 0.3658 | 0.0399 |
| 5E10 | 0.1099 | 0.0304 | 0.1584 | 0.0575 | 0.1675 |
| 5G10 | 0.0835 | 0.1359 | 0.0518 | 0.1365 | 0.0912 |
| 5F8 | 0.0783 | 0.0075 | 0.1019 | 0.0283 | 0.128 |
| 5A7 | 0.0929 | 0.2232 | 0.1762 | 0.2364 | −0.0076 |
a
Binding Response Unit (nm) – in nanometers, measures wavelength shift due to biomolecular binding (reflecting mass changes on the biosensor surface).
b
Paired antibodies are indicated by an underline.
c
The self-pairing or blocking effect, highlighted in gray, is used as a threshold to determine the strong pairs.
d
Mouse-pooled purified IgG was included as a negative control as a detection antibody, and it shows that the sensor is completely saturated.