Fig. 2. Interacting regions between Rb and hDnmt1. (A) Upper panel: the GST–Rb fusion constructs along with the amino acid numbers. Lower panel: the GST pull-down results using purified full-length hDnmt1 protein (+) and anti-N-terminal hDnmt1 antibody. (B) Upper panel: the catalytically active deletion mutants of hDnmt1. Deleted amino acids are indicated on the right. Lower panel: pull-down assay with either GST or GST–Rb ABC is indicated (+), along with the hDnmt1 and mutants at the top.