TABLE 5.
Primers used in this study to detect ESBLs and carbapenemases
| Primer sequence | |||||
|---|---|---|---|---|---|
| Gene | Forward (5' → 3') | Reverse (5' → 3') | Sizea | Annb | Refc |
| bla CTX-M-like | CGATGTGCAGTACCAGTAA | TTAGTGACCAGAATCAGCGG | 585 | 60 | (48) |
| bla GES | CTGGCAGGGATCGCTCACTC | TTCCGATCAGCCACCTCTCA | 600 | 57 | (45) |
| bla PER | AGTGTGGGGGCCTGACGAT | GCAACCTGCGCAATRATAGCTT | 725 | 57 | (45) |
| bla VEB | CGACTTCCATTTCCCGATGC | TGTTGGGGTTGCCCAATTTT | 376 | 57 | (45) |
| bla KPC | TCGCCGTCTAGTTCTGCTGTCTTG | ACAGCTCCGCCACCGTCAT | 353 | 57 | (45) |
| bla NDM | ACTTGGCCTTGCTGTCCTT | CATTAGCCGCTGCATTGAT | 603 | 57 | (45) |
| bla IMI | CTACGCTTTAGACACTGGC | AGGTTTCCTTTTCACGCTCA | 482 | 57 | (47) |
| bla VIM | TGTCCGTGATGGTGATGAGT | ATTCAGCCAGATCGGCATC | 437 | 57 | (45) |
| bla IMP | ACAYGGYTTRGTDGTKCTTG | GGTTTAAYAAARCAACCACC | 387 | 57 | (45) |
| bla OXA-23-like | TACAAGGGATTCGGCATCG | TAATGGCCTGTTCCCATGTG | 570 | 52 | (46) |
| bla OXA-24-like | AAAATCTGGGTACGCAAACG | ACATTATCCGCTGGAACAGG | 271 | 52 | (46) |
| bla OXA-48-like | ATGCGTGTATTAGCCTTATCG | CATCCTTAACCACGCCCAAATC | 265 | 57 | (45) |
| bla OXA-58-like | TCGACACACCTTGGTCTGAA | AACTTCCAACTTTGCCATGC | 477 | 52 | (46) |
a
Size in base pairs (bp).
b
Annealing temperature (°C).
c
References.