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. 2002 Feb 15;21(4):536–545. doi: 10.1093/emboj/21.4.536

Table I. Bacterial strains and plasmids used in this study.

Strain/plasmid Genotype or phenotype Reference
E.coli    
ET8000 rbs lacZ::IS gyrA hutCK Jayakumar et al. (1986)
AT8000 rbs lacZ::IS gyrA hutCK ΔglnB1, ΔamtB Reyes-Ramirez et al. (2001)
FT8000 rbs lacZ::IS gyrA hutCK ΔglnB1, ΔglnK1 Reyes-Ramirez et al. (2001)
GT1000  rbs lacZ::IS gyrA hutCK ΔglnKamtB this work
GT1001 rbs lacZ::IS gyrA hutCK ΔamtB Thomas et al. (2000b)
GT1002 rbs lacZ::IS gyrA hutCK ΔglnK1 this work
PT8000 rbs lacZ::IS gyrA hutCK ΔglnB1 Reyes-Ramirez et al. (2001)
RB9060 ΔlacU169 endA1 thi-1 hsdR17 supE44 hutCK ΔglnB2306 Bueno et al. (1985)
WCH30 ΔlacU169 endA1 thi-1 hsdR17 supE44 hutCK ΩGmr ΔglnK1 Arcondéguy et al. (1999)
YMC10 ΔlacU169 endA1 thi-1 hsdR17 supE44 hutCK Chen et al. (1982)
A.vinelandii    
UW136 Rifr Bishop and Brill (1977)
MV560 Rifr ΔamtB1::KIXX Meletzus et al. (1998)
Plasmids    
pGC2 glnK amtB1 in pACYC184 this work
pGC4 glnK amtB4 in pACYC184 this work
pGT11 glnK amtB in pWSK29 this work
pGT14 amtB in pWSK29 Thomas et al. (2000b)
pMM280 glnK amtB3 in pACYC184 this work
pTA40 glnK in pWSK30 this work
pTA43 glnKY51F in pWSK30 this work