Fig. 1. Activation of FHL2 in response to extracellular stimuli in NIH 3T3 cells. (A) Cells were transfected with the Gal-dependent reporter G5E1b-LUC and expression plasmids for either Gal-FHL2 or the Gal4 DNA-binding domain (Gal) and stimulated with either 10% FCS, 10 µM LPA, 1.2 µM SPP, 50 ng/ml EGF or 50 ng/ml PDGF-BB for 3 h. Cells were serum-starved in 0.5% FCS for 25 h before stimulation. The relative luciferase activity of Gal was set to 1. (B) Stimuli (as in A) were controlled for activity on an SRE-LUC promoter.