Fig. 7. Co-localization of FcRn–GFP and LDL. Bar, 5 µm. Sections labeled as apical, tight junctions, nucleus and basolateral were taken 1, 3, 6 and 9 µm, respectively, from the apical surface of the monolayer. Cells were serum starved and incubated at 37°C with Alexa 646 IgG and DiI-LDL at the apical surface for 30 min in the absence (A) and presence (B) of 150 nM wortmannin. Regions of co-localization appear yellow.