Table II. Total IgG transported or recycled by FcRn–GFP-expressing MDCK cells.
| Picograms of IgG transported at pH 6.0 (per filter) |
Picograms of IgG transported at pH 7.3 (per filter) |
|||||
|---|---|---|---|---|---|---|
| No drug | Wortmannin | Nocodazole | No drug | Wortmannin | Nocodazole | |
| Apical to basolateral | ||||||
| FcRn–GFP | 1924 (42) | 375 (10) | 384 (11) | 1430 (36) | 306 (12) | 333 (9) |
| FcRn | 2526 (51) | 473 (13) | 270 (8) | 1568 (43) | 417 (15) | 350 (12) |
| Basolateral to apical | ||||||
| FcRn–GFP | 353 (9) | 75 (4) | 88 (4) | 330 (4) | 68 (5) | 69 (5) |
| FcRn | 599 (10) | 76 (6) | 110 (6) | 420 (8) | 54 (9) | 85 (8) |
| Apical recycling | ||||||
| FcRn–GFP | 2130 (45) | 1616 (28) | 480 (8) | 1411 (34) | 950 (15) | 385 (12) |
| FcRn | 2461 (45) | 1901 (34) | 360 (12) | 1710 (32) | 1283 (23) | 285 (10) |
| Basolateral recycling | ||||||
| FcRn–GFP | 908 (21) | 147 (5) | 120 (8) | 721 (13) | 101 (3) | 125 (7) |
| FcRn | 1295 (17) | 142 (8) | 130 (15) | 862 (14) | 118 (7) | 105 (6) |
The average of three measurements is shown with the standard deviation in parentheses for each value.