Fig. 2. Aurora-A does not cause centrosome amplification in S phase. HeLa cells were transfected for 48 h with wt or KD mutant EGFP–Aurora-A and cultured in the presence or absence of hydroxyurea (HU). Transfected cells were identified by fluorescence microscopy (A) and the number of centrosomes quantified using the GFP fluorescence of Aurora-A (or anti-C-Nap1 staining; not shown) (B). Histogram shows results from three independent experiments (400–600 cells each) and bars indicate standard deviations. Scale bar: 10 µm.