Table 2.
The details of the protocol used to measure glutathione peroxidase activity.
| Reagent | Test (µl) | Standard (µl) | Control (µl) | Blank (µl) |
|---|---|---|---|---|
| Sample | 10 | 10 | ||
| Glutathione (500 µM) | 100 | 100 | ||
| D.W | 10 | 100 | 110 | |
| Incubation 10 min | ||||
| Peroxide | 40 | 40 | 40 | 40 |
| Incubation 10 min | ||||
| Working solution | 100 | 100 | 100 | 100 |
| Sodium acetate solution | 10 | 10 | 10 | 10 |
| The plate was vortexed, and the absorbance was measured at a wavelength of 570 nm. | ||||