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. 2025 Oct 17;10(1):bpaf075. doi: 10.1093/biomethods/bpaf075

Table 2.

The details of the protocol used to measure glutathione peroxidase activity.

Reagent Test (µl) Standard (µl) Control (µl) Blank (µl)
Sample 10 10
Glutathione (500 µM) 100 100
D.W 10 100 110
Incubation 10 min
Peroxide 40  40  40 40 
Incubation 10 min
Working solution 100  100  100  100 
Sodium acetate solution 10  10  10  10 
The plate was vortexed, and the absorbance was measured at a wavelength of 570 nm.