Table 3.
The details of the protocol used to measure glutathione peroxidase activity.
| Reagents | Test | Control | STD | Blank |
|---|---|---|---|---|
| Sodium phosphate buffer | 1500 | 1900 | 1600 | 2000 |
| Reduced glutathione (2 mM) | 200 | 200 | ||
| Incubation 10 min | ||||
| Sample | 100 | 100 | ||
| The reaction was initiated by adding peroxide: | ||||
| Peroxide | 200 | 200 | ||
| Working solution | 2000 | 2000 | 2000 | 2000 |
| Sodium acetate solution | 100 | 100 | 100 | 100 |
| The test tubes were vortexed, and the absorbance was measured at a wavelength of 570 nm. | ||||