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. 2025 Oct 17;10(1):bpaf075. doi: 10.1093/biomethods/bpaf075

Table 3.

The details of the protocol used to measure glutathione peroxidase activity.

Reagents Test Control STD Blank
Sodium phosphate buffer 1500 1900 1600 2000
Reduced glutathione (2 mM) 200 200 
Incubation 10 min
Sample 100  100 
The reaction was initiated by adding peroxide:
Peroxide 200  200 
Working solution 2000 2000 2000 2000
Sodium acetate solution 100  100  100  100 
The test tubes were vortexed, and the absorbance was measured at a wavelength of 570 nm.