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. 2025 Nov 5;30:1067. doi: 10.1186/s40001-025-03343-3

Table 2.

Embryo parameters among the different groups

Characteristics  < 7-cell 7–9-cell 10–13-cell  > 13-cell P-value
Number of cycles 110 743 282 84
Evenness of day 3 embryo (%)  < 0.001
 0 6.36 (7/110) 22.75 (169/743)a 12.41 (35/282)b 21.43 (18/84)a
 1 85.45 (94/110) 73.76 (548/743)a 86.17 (243/282)b 78.57 (66/84)
 2 8.18 (9/110) 3.50 (26/743) 1.42 (4/282)a 0.00 (0/84)a
Fragmentation of day 3 embryo (%)  < 0.001
  < 10% 23.64 (26/110) 40.51 (301/743)a 52.13 (147/282)a 50.00 (42/84)a
 10–25% 43.64 (48/110) 53.57 (398/743) 44.33 (125/282) 47.62 (40/84)
  > 25% 32.73 (36/110) 5.92 (44/743)a 3.55 (10/282)a 2.38 (2/84)a
Expansion grade (%) 0.325
 IV 45.45 (50/110) 46.57 (346/743) 38.65 (109/282) 39.29 (33/84)
 V 50.00 (55/110) 48.59 (361/743) 55.32 (156/282) 57.14 (48/84)
 VI 4.55 (5/110) 4.85 (36/743) 6.03 (17/282) 3.57 (3/84)
Grade of transferred blastocysts (%)  < 0.001
 High-quality 66.36 (73/110) 86.27 (641/743)a 93.97 (265/282)ab 97.62 (82/84)ab
 Low-quality 33.64 (37/110) 13.73 (102/743) 6.03 (17/282) 2.38 (2/84)
embryonic development days (%)  < 0.001
 Day 5 42.73 (47/110) 78.33 (582/743)a 91.49 (258/282)ab 98.81 (83/84)ab
 Day 6 57.27 (63/110) 21.67 (161/743) 8.51 (24/282) 1.19 (1/84)

1. P value indicates differences among the four groups. P < 0.05 indicates statistically significant difference in results

2. a indicates a significant difference compared to < 7-cell group; b indicates a significant difference compared to 7–9-cell group. According to Bonferroni correction for multiple comparisons, the difference was statistically significant at P < 0.008 (0.05/6)