Fig. 6. K-Ras is required for OSM-triggered E-cadherin localization. K-Ras^–/– fetal hepatocytes were infected on day 0 with the pMIG retroviral vector carrying no signaling molecule (A), K-Ras (B), H-Ras (C) or N-Ras (D), and cultured for 6 days with OSM/Dex. To investigate their effect on OSM-triggered E-cadherin localization, subcellular localization of E-cadherin was analyzed (middle panel). Left panel: fluorescence of GFP. Right panel: merged image. E-cadherin was highly concentrated at cell–cell junctional sites (arrows) in cells infected with K-Ras virus (B). Scale bar: 10 µm. (E) Ectopic expression of the Ras family proteins in fetal hepatocytes. Wild-type hepatocytes were infected with the pMIG vector carrying a Ras cDNA and cultured for 6 days with OSM/Dex. Cell lysates from each culture were subjected to western blotting using antibodies against K-Ras, H-Ras, N-Ras or STAT3 (internal control).