Table I. List of proteins affected by TGFβ1 and identified by PMF.
| Spot | Protein | Probability | Est’d Z | Sequence coverage (%) | NCBI ID No. or accession No. | Theoretical value |
Experimental value |
Fold changes |
|||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pI | Mr (kDa) | pH | Mr (kDa) | 2 h | 24 h | ||||||
| S1 | Tax interaction protein 43 | 8.6E–01 | 1.07 | 19 | AF028828 | 9.2 | 31.8 | 8.7 | 31 | 1.04 | 0.50 |
| S2 | elastase 1 precursor | 9.7E–01 | 0.62 | 22 | AF120493 | 9.2 | 27.8 | 7.7 | 26 | 1.56 | 0.85 |
| S3 | transcriptional factor TFEC | 6.0E–01 | 1.03 | 10 | AF077742 | 6.3 | 35.1 | 6.9 | 40 | 1.20 | 0.62 |
| S4 | isopropylmalate isomerase | 6.8E–01 | 0.29 | 22 | gi/3122365 | 7.0 | 22.5 | 6.5 | 29 | 1.46 | 0.72 |
| S5 | anti-oxidant protein 2 | 8.9E–01 | 0.57 | 45 | gi/6671549 | 6.0 | 24.8 | 6.4 | 31 | 0.88 | 0.66 |
| S6 | collagenase-3 precursor | 9.1E–01 | 0.47 | 18 | gi/116872 | 5.1 | 53.4 | 6.2 | 32 | 0.43 | 0.47 |
| S7 | cytosolic inorganic pyrophosphatase | 1.0E+00 | 1.55 | 29 | AF108211 | 5.4 | 31.8 | 6.2 | 35 | 0.99 | 0.65 |
| S8 | caspase 3 | 9.7E–01 | 0.63 | 19 | gi/2493528 | 6.2 | 32.1 | 6.1 | 32 | 0.98 | 0.47 |
| S9 | keratin 10, type 1, cytoskeleton | 1.0E+00 | 1.40 | 16 | gi/106849 | 4.7 | 39.7 | 5.9 | 30 | 1.37 | 0.81 |
| S10 | apolipoprotein-A1 | 9.5E–01 | 0.52 | 23 | U79574 | 5.5 | 30.5 | 5.7 | 26 | 1.13 | 0.75 |
| S11 | hypothetical protein | 1.0E+00 | 0.90 | 20 | gi/7705535 | 5.3 | 36.0 | 5.6 | 30 | 0.52 | 0.65 |
| S12 | GFAP | 6.9E–01 | 1.37 | 16 | gi/223869 | 6.0 | 24.9 | 5.5 | 21 | 1.59 | 0.58 |
| S13 | protein kinase C inhibitor | 1.0E+00 | 1.92 | 16 | gi/284655 | 4.8 | 27.8 | 5.0 | 31 | 1.33 | 0.84 |
| S14 | caspase 1 | 7.6E–01 | 0.53 | 20 | AF090119 | 6.1 | 45.3 | 5.2 | 26 | 0.38 | 0.44 |
| S15 | guanidine nucleotide binding protein, α-inhibiting 2 | 7.2E–01 | 1.37 | 21 | gi/6680037 | 5.4 | 40.5 | 5.1 | 32 | 1.14 | 0.72 |
| S16 | RAG1 | 7.6E–01 | 1.38 | 16 | AF203759 | 5.7 | 42.9 | 5.0 | 35 | 1.26 | 0.78 |
| S17 | Son of sevenless 1 | 4.7E–01 | 0.73 | 28 | D83014 | 4.9 | 23.6 | 4.7 | 20 | 1.12 | 0.69 |
| S18 | Musashi-1 homolog | 6.5E–01 | 0.28 | 11 | gi/6678940 | 7.9 | 39.1 | 8.2 | 43 | 1.13 | 2.41 |
| S19 | phosphoglycerate kinase-1 | 1.0E+00 | 2.43 | 15 | gi/104957 | 8.4 | 44.8 | 7.6 | 43 | 2.22 | 2.25 |
| S21 | ubiquitin-like fusion protein An1b | 5.7E–01 | 0.93 | 12 | gi/48443 | 8.6 | 78.6 | 7.6 | 100 | 1.10 | 2.54 |
| S22 | far upstream element-binding protein | 1.0E+00 | 1.33 | 12 | gi/4503801 | 7.2 | 67.5 | 7.6 | 98 | 0.98 | 2.23 |
| S23 | inosine-5′-monophosphate dehydro genase 2 | 1.0E+00 | 2.32 | 21 | gi/124419 | 6.4 | 55.8 | 7.2 | 55 | 0.76 | 0.68 |
| S24 | lamin A | 1.0E+00 | 1.05 | 16 | X76297 | 6.2 | 71.6 | 7.0 | 110 | 1.42 | 1.44 |
| S25 | indole-3-acetaldehyde dehydrogenase | 9.2E-01 | 0.57 | 17 | U74468 | 6.1 | 53.7 | 6.0 | 62 | 0.99 | 0.57 |
| S26 | caspase 10 | 1.0E+00 | 1.11 | 13 | AB038173 | 5.6 | 59.6 | 5.9 | 40 | 1.07 | 1.43 |
| S27 | p59 protein | 9.9E–01 | 0.68 | 17 | gi/122768 | 5.3 | 51.5 | 5.8 | 55 | 1.65 | 2.53 |
| S28 | type 2 keratin subunit protein | 1.0E+00 | 1.49 | 22 | M10938 | 5.3 | 52.8 | 5.4 | 42 | 1.22 | 1.50 |
| S29 | keratin 10 | 1.0E+00 | 2.43 | 15 | gi/4557697 | 5.0 | 57.2 | 5.4 | 48 | 2.39 | 1.19 |
| S30 | calreticulin | 9.6E–01 | 1.45 | 20 | gi/237420 | 4.3 | 46.3 | 4.6 | 70 | 0.81 | 1.34 |
| R1 | 67K cytoskeletal type 2 keratin | 9.9E–01 | 0.84 | 15 | gi/88054 | 6.0 | 65.5 | 5.9 | 100 | 0.89 | 0.41 |
| R2 | hnRNP L | 9.6E–01 | 0.72 | 10 | gi/4557645 | 6.7 | 60.2 | 7.2 | 70 | 1.17 | 0.59 |
| R3 | HSP60 | 1.0E+00 | 2.35 | 22 | X53585 | 5.3 | 57.9 | 5.6 | 65 | 1.10 | 0.68 |
| R4 | enolase-1 | 9.9E–01 | 1.55 | 20 | gi/4503571 | 7.0 | 47.2 | 6.8 | 50 | 1.54 | 1.01 |
| R5 | RAD51 | 1.0E+00 | 2.40 | 17 | gi/4506389 | 5.3 | 37.0 | 5.2 | 38 | 1.10 | 0.68 |
| R6 | tropomyocin α-chain | 1.0E+00 | 2.43 | 14 | gi/112448 | 4.9 | 32.1 | 5.0 | 35 | 0.95 | 1.58 |
| R8 | adducin | 9.9E–01 | 0.88 | 21 | gi/321247 | 8.8 | 20.3 | 8.4 | 22 | 1.16 | 0.83 |
| R9 | peptidylprolyl isomerase A | 1.0E+00 | 2.43 | 27 | gi/68401 | 8.8 | 17.7 | 8.6 | 18 | 1.31 | 0.76 |
| R10 | myosin regulatory light chain | 4.8E–01 | 0.53 | 43 | X54617 | 4.6 | 6.7 | 5.4 | 15 | 1.67 | 1.08 |
S1–30, silver-stained spots; R1–10, radioactively labelled spots; gi, NCBI sequence identification numbers.
S20 and R7 did not provide reliable results. Probability, Est’d Z, coverage and theoretical pI and Mr were obtained from the Pro Found search.
The calculation of experimental pI and Mr was based on migration of the protein on a 2D gel.
Fold changes presents the ratio compared with values in the absence of TGFβ1 stimulation, taken as 1.0.