Appendix 3—table 1. Annotation of the chemical compounds significantly associated with both sexes of M. helenor or M. achilles using an Indicator value analysis.
We used an Indicator Value analysis to find the chemical compounds allowing us to discriminate the males and females of M. helenor and M. achilles separately. Because we used a protocol allowing for the detection of small peaks during the MZmine analysis to not exclude ‘pheromone-like’ molecules, our analysis is sensitive to small spectral variations. This could explain why some compounds were associated with different (but similar) MZmine-detected occurrences. X refers to an undetermined double bond position or configuration (Z or E). The ‘Ions’ column describes the spectrum with the major ion, followed by other ions in intensity order, and the underlined visible ion corresponding to the molecular ion.
| Sex | Dataset | Annotation | LRI | Ions | Species | p-value |
|---|---|---|---|---|---|---|
| Males | C8 to C16 | (E)-β-Ocimene | 1048 | 93; 79; 41; 136 | M. helenor | 0.0491 |
| Phenyl ethyl alcohol | 1112 | 91; 122; 65 | M. achilles | 0.0004 | ||
| Unknown compound 1 | 1123 | 80; 52; 73; 98; 124 | M. helenor | 0.0311 | ||
| Ethyl octanoate | 1195 | 88; 57; 101; 43; 73; 127; 172 | M. helenor | 0.0001 | ||
| Tetradec-1-ene | 1390 | 43; 55; 69; 83; 97; 196 | M. achilles | 0.0282 | ||
| Ethyl decanoate | 1394 | 88; 101; 43; 73; 155; 200 | M. helenor | 0.0001 | ||
| Dodec-x-enol | 1454 | 55; 68; 41; 82; 96; 184 | M. helenor | 0.0002 | ||
| Ethyl dodecanoate | 1593 | 88; 101; 43; 73; 155; 228 | M. helenor | 0.0001 | ||
| (x)-Ethyl Tetradec-x-enoate | 1764 | 88; 55; 41; 96; 166; 254 | M. helenor | 0.0001 | ||
| (x)-Tetradec-x-enyl acetate | 1784 | 43; 68; 54; 82; 96; 194 | M. helenor | 0.0001 | ||
| C16 to C30 | Ethyl hexadecanoate | 1993 | 88; 101; 43; 73; 155; 284 | M. helenor | 0.0001 | |
| Unknown compound 2 | 2046 | 44; 207; 49; 83; 55; 69 | M. helenor | 0.0001 | ||
| Geranyl decanoate | 2148 | 69; 93; 41; 121; 136; 308 | M. helenor | 0.0001 | ||
| (Z)-Ethyl Octadec-9-enoate | 2168 | 55; 41; 69; 88; 83; 310 | M. helenor | 0.0001 | ||
| Ethyl octadecanoate | 2193 | 88; 101; 43; 55; 157; 312 | M. helenor | 0.0001 | ||
| Unknown compound 3 | 3109 | 44; 207; 57; 71; 85; 281 | M. achilles | 0.0096 | ||
| (x)-Tetradec-x-enyl hexadecanoate | 3148 | 68; 82; 96; 194; 43; 450 | M. helenor | 0.0001 | ||
| Unknown compound 4 | 3342 | 207; 43; 55; 73; 81; 95 | M. achilles | 0.0159 | ||
| (x)-Tetradec-x-enyl octadecanoate | 3350 | 68; 82; 96; 194; 43; 57 | M. helenor | 0.0001 | ||
| Females | C8 to C16 | Dec-1-ene | 990 | 56; 41; 70; 83; 97; 140 | M. helenor | 0.028 |
| Dodec-1-ene | 1190 | 43; 55; 69; 83; 97; 168 | M. helenor | 0.0302 |