Table I. Quantitation of northern blot analysis.

Strain	Mature tRNATrp	Precursor tRNATrp	Total tRNATrp	Precursor form (%)	Total ts-tRNA/wt-tRNA (%)
wt-tRNATrp
wild type	310	9	319	<5
PAP–	263	17	280	6
PNP–	404	5	409	<5
PAP–PNP–	374	14	388	<5
ts-tRNATrp
wild type	44	3	47	7	15
PAP–	50	32	82	39	29
PNP–	121	12	133	9	33
PAP–PNP–	104	121	225	54	58

Individual bands from Figure 1 were analyzed with a PhosphorImager for quantitation. Data are expressed in arbitrary units that have been normalized to the 5S rRNA content of each lane. The experiment presented here is typical of multiple experiments with similar results. However, because the absolute numbers vary greatly from experiment to experiment due to the northern analysis and PhosphorImager quantitation, we have not presented standard errors.