Fig. 3. Localization of MSL2 is disrupted in males lacking both roX RNAs. (A and B) Wild-type male. (C and D) Male mutated for roX1 (roX1ex6). (E and F) Male deleted for roX2 (Df(1)52/Y; [4Δ4.3]/+). (G and H) Male mutated for both roX RNAs (roX1ex6 Df(1)52/Y; [4Δ4.3]/+). (A, C, E and G) Chromosome preparations counter stained with Hoechst 33258. (B, D, F and H) Chromosome preparations probed with antibodies against MSL2 and detected with Texas Red. In (G and H) the arrowhead points to the fourth chromosome; in (H) the arrow indicates the chromocenter and asterisks mark two strongly staining autosomal sites of MSL2 localization. ‘X’ indicates the X chromosome. Exposure times for Texas Red were between 6 and 8 s.